Technology in medicine has taken a new dimension. With regards to testing for presence of diseases and other anomalies, Elisa technology has taken over. The cardiac Elisa kits have been particularly so good. They are devices capable of working with the hearts of almost all animals in the world to establish any defects on it.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This process can be used in establishing the presence of foreign bodies in human beings. It is very important since it helps in detecting and treating heart problems before they develop into serious problems. This helps in cutting down the cost involved in diagnosing and treating heart defects. This is because the defects are discovered in their early stages before they become serious problems.
The best of these tools is one that is accurate, sensitive and capable of working in a wide range of samples. Sensitivity means being able to detect and show slight changes in the reagents. Accuracy, on the other hand, is being free of major errors in making measurements. It is also very important for the instrument to be made in a way such that, each heart defect has its own diagnostic equipment.
It is also important that the instruments are made in a way that makes them stable. To attain stability, one must cut down on the rate loss of these activities. This is possible through proper storage. Stability can also be achieved through minimizing the effects of the surrounding on the set-up. This means temperature, humidity and pressure have to agree with the standard lab requirements. There should be somebody to control incubator temperatures. If only one person is allowed to work on the research from beginning to end, it will be easy to achieve stability.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
The process of diagnosing illnesses depends on an enzyme reaction that gives its results through exhibiting certain color changes. The enzyme and some antibodies combine with antigens, and subsequently change their color to indicate occurrence of a reaction. With this test, researchers are able to detect both antigens and antibodies.
This process can be used in establishing the presence of foreign bodies in human beings. It is very important since it helps in detecting and treating heart problems before they develop into serious problems. This helps in cutting down the cost involved in diagnosing and treating heart defects. This is because the defects are discovered in their early stages before they become serious problems.
The best of these tools is one that is accurate, sensitive and capable of working in a wide range of samples. Sensitivity means being able to detect and show slight changes in the reagents. Accuracy, on the other hand, is being free of major errors in making measurements. It is also very important for the instrument to be made in a way such that, each heart defect has its own diagnostic equipment.
It is also important that the instruments are made in a way that makes them stable. To attain stability, one must cut down on the rate loss of these activities. This is possible through proper storage. Stability can also be achieved through minimizing the effects of the surrounding on the set-up. This means temperature, humidity and pressure have to agree with the standard lab requirements. There should be somebody to control incubator temperatures. If only one person is allowed to work on the research from beginning to end, it will be easy to achieve stability.
Before the experiment is done, the researcher must prepare all the standards, samples and reagents. Some samples are then added to each well and incubated for approximately two hours. Having done this, the researcher should then aspire the previous mixture before adding a small amount of the reagent. He/she must then incubate the mixture for one hour. The substances are once again aspired and washed three times before a solution of the substrate is added and then incubated for 20-25 minutes. Lastly, a stopping solution is added to end the reaction.
This process applies the sandwich enzyme principle. The plate that comes with the kit is coated with antibodies that are specific to the defect to be diagnosed. Standards/samples are later put on the plate as is appropriate. The samples or standards contain biotin-conjugate antibodies that are specific for the defect. Avidin conjugate is then added to every plate before incubating.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
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