Medical science is advancing in leaps and bounds. New treatments, drugs and procedures are introduced regularly. One of the main areas of studies remains the development of new tests that will help physicians to diagnose diseases quicker. The earlier a condition is diagnosed the bigger the chance that it can be treated successfully. ELISA kits have done much to make the diagnosis of several conditions both easier and quicker.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
Diagnosticians can get a lot of information form even the most basic EIA test. However, there are several versions or formats of the test and some are much more complex. The direct EIA, for example, requires that the antigen is screened with a multiwall coat. This makes it more difficult for the antibody to recognize the antigen. A result can only be obtained if the antibody is joined with a specific enzyme.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
Patients need not fear this simple test. It only entails the drawing of a small blood sample. This sample is then added to specific antigens in a petri dish. If there is a reaction between the blood sample and the antigen, then the presence of antibodies is confirmed. In fact, the antibodies will combine with the antigens. By adding enzymes it is possible to determine the amount of the antibodies in the sample.
Diagnosticians can get a lot of information form even the most basic EIA test. However, there are several versions or formats of the test and some are much more complex. The direct EIA, for example, requires that the antigen is screened with a multiwall coat. This makes it more difficult for the antibody to recognize the antigen. A result can only be obtained if the antibody is joined with a specific enzyme.
The indirect IEA test is another example of how this protocol can be used in an advanced manner. In this instance the antigen is actually covered by a polystyrene plate. This forces the antibodies to go through two distinct stages before the antigen is detected. This test allows technicians to identify both primary and secondary antibodies. This is valuable because it may eliminate the need for further expensive tests.
In the case of yet another type of EIA test, the sandwich test, pairs of antibodies are tested. The first antibody is covered with a polystyrene plate. The next antibody is then added in order to determine the concentration of the analyte. This is a complicated procedure but it is extremely accurate and flexible.
Nobody will deny the fact that diseases are easier and cheaper to treat if they can be diagnosed at an early stage. Tests such as the EIA help doctors to do just this. Conditions such as HIV, pernicious anemia, syphilis, and many others have been diagnosed early enough to save the lives of the patient, all thanks to this amazing procedure.
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